ATP Qadenosiue tgphghatel, studies on neutrophils
ATP is hydrolysed to ADP and phosphate as the major energy source in
muscle and other tissues. lt is regenerated by
oxidative phosphorylation of ADP in the mitochondria. When aerobic
metabolism provides insuficient energy, extra
ATP is generated during the anaerobic breakdown of glucose to lactic
acid. ATP reactions require magnesium. ADP to
ATP conversion can be blocked by em/ironmullal contaminants as can the
translocator ['l"L] in the mitochondrial
membrane. [TLI efftciericy is also sensitive to pH and other
metabolic-factor changes. [TL] defects may demand
excessive ADP to AMP conversion (not re-converted to ADP or through to
A1'P). Defects in Mg-ATP, ADP - ATP
conversion and enzyme or [TL] blocking can all result in ehrolle fatigue
- a factor ln any disease where
biochemical energy availability ts reduced.
ATP whole cells:
With excess Mg added 1,89 nmol/ l0° cells 1.6 - 2.9
(Standard method of measuring ATP)
Endogenous Mg only 1.27 nmol/10° oeus 0.9 - 2.7
(Meastred ATP result is lowered during intracellular magnesium
deficiency)
Ratio ATP/A1'P““ 0.67 ...... ................... > 0.65
ADP to ATP conversion @cien (whole cells):
A (from above) 1,89 nmol/l0° cells (lr) 1.6 - 2.9
ATP” (inhibitor present) 0,13 nmol/10° cells (2*) < 03
ATPM' (inhibitor removed) 1.37 nmol/l0°cells (3*) >1.4
Am' to ATP ornaoooy [(3*- 2*)/(l*- 2~)} x loo = 70.5 % > so
Blocking of active sites (2*/l*) x l00 = 6.9 % up to 14
ADP-ATP TRANSLOCATQR [TL] (mitochondria, not whole cells):
ATP Ref. range chgge % ret range
(pmol/IB* cells)
Start 396 290 - 700
[TL] ‘out’ 572 410 - 950 44,4 over 35% (Increase)
(in-vitro test) reflects ATP supply for cytoplasm
[TL] ‘in’ 187 140 - 330 523 55 to 75% (Decrease)
(in-vitro test) rctlects nonnal use of ATP on energy demand
Normal whole-cell ATP ATP-related Mg seems to be normal.
No significant blocking of active sites. ADT-ATP is nonnal but slightly
slow.
Nomial mitochondrial function.
Excellent progress.
The table above includes all adducts fond. The following is a list of
common adducts
(Included In the above table If found In this sample).
Clemleal or grelpc Aminee-gmenl. Diemino compounds, Diem compounds,
Nltrosaminea.
compounds, Vinyl halide, Acrylemlde, Melonmdhldehyde,
Other eldehydee, Anwoxln/mimoxlns, Lindane (& other Bl-ICs), Tcxsphme,
Od: ocgmochlorlne
compounds, Other argmophoeplumes, metabolites, Phlhelnee,
proteins, ovme peptides.
Metals: Aluminium, Antimony, Arsenic, Barium, Cadmium, Chromium, Cobalt,
Coppe, Lead, Manganese,
Mecury, Nickel, Platinum, Rhodium, Silver, Strontium, ThaIlium, Tin,
Titanium.
Trefwoorden:
Omschrijving Normaalwaarde
Verterlng
pl-I - s.a
Consistentie Vast
Kleur Bruin
Zetmeelvezels Geen
Plantvezels
Vetgehalte
Kolonievormende eenheden per gram ontlasting
Enterobacteriaceae spp.
E.coli
Enterococcus spp.
Lactobacillus spp.
Bifidobacterlum spp.
Gisten Geen
Schimmels Geen
Immunologische infectie- en ontstekingsmarkers
secretoír IgA
soo - H9/ml
1gA Antigliadine
IgA anti-t-Transglutaminase <= U/I
TNF P9/ml
Calprotectlne mg/kg
Parasltologle
Giardia lamblia Negatief
Cryptosporldlum spp. Negatief
Opmerkingen:
Uitslag aangegeven met * duidt op een afwijkende waarde.
Resultaat
Gasvorming
Lichtbruin
S1
Negatief